Prospects for biodiversity conservation in the Atlantic Forest: Lessons from aging human-modified landscapes   总被引：1，自引：0，他引：1  

Marcelo Tabarelli  Antonio Venceslau Aguiar  Jean Paul Metzger 《Biological conservation》2010,143(10):2328-2340

Recent global assessments have shown the limited coverage of protected areas across tropical biotas, fuelling a growing interest in the potential conservation services provided by anthropogenic landscapes. Here we examine the geographic distribution of biological diversity in the Atlantic Forest of South America, synthesize the most conspicuous forest biodiversity responses to human disturbances, propose further conservation initiatives for this biota, and offer a range of general insights into the prospects of forest species persistence in human-modified tropical forest landscapes worldwide. At the biome scale, the most extensive pre-Columbian habitats across the Atlantic Forest ranged across elevations below 800 masl, which still concentrate most areas within the major centers of species endemism. Unfortunately, up to 88% of the original forest habitat has been lost, mainly across these low to intermediate elevations, whereas protected areas are clearly skewed towards high elevations above 1200 masl. At the landscape scale, most remaining Atlantic Forest cover is embedded within dynamic agro-mosaics including elements such as small forest fragments, early-to-late secondary forest patches and exotic tree monocultures. In this sort of aging or long-term modified landscapes, habitat fragmentation appears to effectively drive edge-dominated portions of forest fragments towards an early-successional system, greatly limiting the long-term persistence of forest-obligate and forest-dependent species. However, the extent to which forest habitats approach early-successional systems, thereby threatening the bulk of the Atlantic Forest biodiversity, depends on both past and present landscape configuration. Many elements of human-modified landscapes (e.g. patches of early-secondary forests and tree monocultures) may offer excellent conservation opportunities, but they cannot replace the conservation value of protected areas and hitherto unprotected large patches of old-growth forests. Finally, the biodiversity conservation services provided by anthropogenic landscapes across Atlantic Forest and other tropical forest regions can be significantly augmented by coupling biodiversity corridor initiatives with biota-scale attempts to plug existing gaps in the representativeness of protected areas.  相似文献   

Development of an indirect enzyme-linked immunosorbent assay for the detection of leptospiral antibodies in dogs.   总被引：2，自引：0，他引：2       下载免费PDF全文

M J Ribotta  R Higgins  M Gottschalk    R Lallier 《Canadian journal of veterinary research》2000,64(1):32-37

Serology plays an important role in the diagnosis of leptospirosis. Few laboratories have the resources, expertise, or facilities to perform the microscopic agglutination test (MAT). Thus, there is a need for a rapid and simple serological test that could be used in any diagnostic laboratory. In this study, a genus-specific, heat-stable antigenic preparation from Leptospira interrogans serovar pomona was used in an enzyme-linked immunosorbent assay (ELISA) for the detection of leptospiral antibodies in dog sera. This antigenic preparation reacted with rabbit antisera against L. interrogans serovars bratislava, autumnalis, icterohaemorrhagiae and pomona and with rabbit antiserum against L. kirschneri serovar grippotyphosa. The ELISA showed a relative specificity of 95.6% with 158 dog sera which were negative at a dilution of 1:100 in the MAT for serovars pomona, bratislava, icterohaemorrhagiae, autumnalis, hardjo, and grippotyphosa. The relative sensitivity of this assay with 21 dog sera that revealed serovars MAT titres of > or =100 to different serovars was 100%. This assay is easily standardized, technically more advantageous than MAT, and uses an antigenic preparation that can be routinely prepared in large amounts. It was concluded that this ELISA is sufficiently sensitive test to be used as an initial screening test for the detection of leptospiral antibodies in canine sera, with subsequent confirmation of positive test results with the MAT.  相似文献   

Gastric coccidiosis in Thoracocharax stellatus caused by Goussia guamaensis n. sp. (Apicomplexa: Eimeriidae) from the Amazon region     

Marcelo F. da Silva  Rebeca S. de Araújo  Diehgo T. da Silva  Osimar Sanches  Igor Hamoy  Edilson Matos 《Journal of fish diseases》2019,42(6):905-912

Ninety‐seven specimens of spotfin hatchetfish, Thoracocharax stellatus, an ornamental freshwater species from the Amazon basin, were captured in the basin of the Guamá River in the municipality of Belém, in northern Brazil, and analysed for coccidiosis infection. Overall, 26 of the specimens were infected by apicomplexan parasites of the genus Goussia, with unsporulated forms being found in the gastric epithelium and sporulated forms in the intestinal lumen. The spheroid oocysts (mean diameter: 13.2 ± 1.7 μm) have four elliptical sporocysts. A partial sequence of the SSU rDNA of the new species was obtained, which contained 1,121 base pairs, with 43.8% guanine + cytosine (G + C), and the bases distributed as follows: A = 28.1%, C = 18.3%, G = 25.5% and T = 28.1%. The combined analysis of the morphometric and phylogenetic evidence confirmed that the specimens represented the genus Goussia and were allocated to a new species, Goussia guamaensis n. sp., which is described here.  相似文献   

Agronomic,nutritive value,reproductive, cytogenetic,and molecular aspects of Paspalum accessions: Contribution to the development of new forage cultivars     

Frederico de Pina Matta  Alessandra Pereira Fávero  Bianca Baccili Zanotto Vigna  Marisa Toniolo Pozzobon  Sergio Raposo de Medeiros  Waldomiro Barioni Júnior  Marcelo Mattos Cavallari 《Grass and Forage Science》2023,78(1):101-118

This study investigated the agronomic, nutritive value, cytogenetic, molecular, and reproductive aspects of twenty-five Paspalum accessions to support breeding programs. The agronomic aspects and the nutritive value were evaluated in a randomized block experimental design with four replications, and with monthly repeated measures for 2 years. For the molecular characterization 16 microsatellite markers developed for different Paspalum species in previous works were used. The number of chromosomes was determined by visualizing the meiotic cells and the DNA content was determined by flow cytometry. The embryo sac clarification technique was used to characterize the reproductive system. T he results indicated genetic variability for all the variables related to yield and nutritive value. For almost all variables, the values of Paspalum accessions were higher than those of the commercial cultivars Megathyrsus maximus cv. Tanzania and Urochloa brizantha cv. Marandu, used as references. However, the accessions showed in vitro dry matter digestibility values lower than the mean value presented by the references. The groups formed in the molecular analysis showed correspondence to the groups observed in the principal components analysis, considering the variables related to yield and nutritive value. Most accessions were revealed to be tetraploid with 40 chromosomes and most accessions belonging to the Virgata group displayed a sexual embryo sac structure . Some crosses can be indicated for genetic breeding, due to a superior genomic affinity between the accessions and the higher probability of heterosis in the progeny. These results provide subsidies to genetic plant breeding programs that use these accessions.  相似文献   

Variability in the solubility of agricultural limestone from different sources and its pertinence for aquaculture          下载免费PDF全文

Marcelo Vinicius do Carmo e Sa  Claude E Boyd 《Aquaculture Research》2017,48(8):4292-4299

Samples of agricultural limestone were obtained from quarries in the USA as follows: Talking Rock, GA; Whitestone, GA; Austinville, VA; Bonham, TX; and Thomasville, PA, referred as GA‐1, GA‐2, VA, TX and PA respectively. The limestone products were tested initially for non‐equilibrium pH, concentrations of calcium and magnesium, neutralizing value (NV) and fineness rate. Laboratory solubility test of the products was conducted in which pH, specific conductance (SC), total alkalinity (TA), total and calcium hardness were measured weekly for 9 weeks. It was found that the agricultural limestone samples had comparable chemical compositions, that is NV (97–108%), Ca (19.8–32.1%), Mg (3.2–12.2%), pH of slurry (8.1–9.7), but somewhat different solubilities in water. Total alkalinity concentrations at equilibrium ranged from 30 to 60 mg L^?1. It was possible to quickly estimate the relative solubility of different agricultural limestone samples by comparing their specific conductance under standardized conditions (2.0 g samples w/0.15–0.25 mm fineness; 3.5 L distilled water; 24 h). This procedure might be helpful in achieving better results when liming aquaculture ponds, that is choosing the agricultural limestones with the highest solubilities in water.  相似文献   

Early development of the silver catfish Rhamdia quelen (Quoy & Gaimard, 1824) (Pisces:Heptapteridae) from the São Francisco River Basin, Brazil     

Marcelo Pimenta de Amorim  Bruno Vilaça Campos Gomes  Yuri Simões Martins  Yoshimi Sato  Elizete Rizzo  & Nilo Bazzoli 《Aquaculture Research》2009,40(2):172-180

The silver catfish, Rhamdia quelen , is endemic to North, Central and South America with high aquaculture potential and wide acceptance in the market. Breeder fish were subjected to induced reproduction through hypophysation using a crude common carp pituitary extract. Egg characteristics, oocyte surface ultrastructure and histology of larval ontogenesis until whole yolk resorption were described for the first time for this species. Oocytes and semen were obtained by manual extrusion, and fertilization was conducted using the dry method. After fertilization, eggs were kept in incubators at 24 °C. The embryonic development was monitored using a stereomicroscope every 10 min until hatching. To analyse the larval development, larvae samples were collected from incubators daily until the fifth day, fixed in Bouin's fluid and subjected to routine histological techniques. The oocyte extrusion occurred 8 h after the second hormone dose at 26 °C. The oocytes were spherical, non-adhesive and yellow, with a diameter of 1471.75±47.63 μm. Scanning electron microscopy revealed a thin jelly coat covering the zona radiata in the animal pole around the micropyle. The blastopore closure occurred within 8 h after fertilization, and the fertilization rate was 79.9±5.2% at 24 °C. Embryonic development was completed within 25 h 30 min after fertilization. The complete resorption of the yolk and the formation of the digestive system organs and the mouth opening occurred on the fifth day, indicating a need for exogenous feeding. The results of this study provide information important for improvement in R. quelen culture and management.  相似文献   

Detection of Bemisia tabaci Mediterranean cryptic species on soybean in São Paulo and Paraná States (Brazil) and interaction of cowpea mild mottle virus with whiteflies     

Vinicius Henrique Bello  Felipe Barreto da Silva  Luís Fernando Maranho Watanabe  Eduardo Vicentin  Cristiane Muller  Regiane Cristina Oliveira de Freitas Bueno  Jessica Costa Santos  Bruno Rossitto De Marchi  Angélica Maria Nogueira  Valdir Atsushi Yuki  Julio Massaharu Marubayashi  Maria Márcia Pereira Sartori  Marcelo Agenor Pavan  Murad Ghanim  Renate Krause-Sakate 《Plant pathology》2021,70(6):1508-1520

Since the detection of Bemisia tabaci Middle East-Asia Minor 1 (MEAM1) species in Brazil in the early 1990s, this whitefly has been the predominant species and the main viral vector in the country. In this work, whiteflies were collected from commercial soybean fields near and far from greenhouses where Mediterranean (MED) species had previously been detected infesting vegetable crops. Results indicated that MEAM1 was the predominant whitefly species in soybean in most sampling sites, while MED was found colonizing soybean plants in open field conditions alone and/or with MEAM1 in several places. Among the tested insects, MED species was mostly detected harbouring the facultative endosymbiont Hamiltonella. We also detected cowpea mild mottle virus (CPMMV) infecting soybean plants. Transmission assays demonstrated that MED species was more efficient transmitting CPMMV from beans to beans and from soybean to soybean plants than MEAM1, while MEAM1 was more efficient in transmitting the virus from soybean to bean than MED. Performance assays indicated that adult emergence of both species on CPMMV-infected soybean plants was higher when compared with the emergence on healthy plants. Moreover, nonviruliferous MED and MEAM1 adults preferred to settle more often on healthy plants, while viruliferous adults settled more often on CPMMV-infected soybean plants. As MED has already been found in soybean open fields in São Paulo and Paraná States, Brazil, and it is a good vector of CPMMV, we anticipate problems related to whitefly management and to increase in the incidence of the virus in soybean.  相似文献   

Do fish isotopic niches change in an Amazon floodplain lake over the hydrological regime?     

Lucas Silva Azevedo  Incio Abreu Pestana  Marcelo Gomes Almeida  Wanderley Rodrigues Bastos  Cristina Maria Magalhes Souza 《Ecology of Freshwater Fish》2022,31(1):72-80

Floodplain lakes are ecosystems characterised by annual flood and dry cycles. Fish ecology is influenced by the flood pulse due to the large influx of allochthonous food resources and diversification of habitats during the flood cycle, while during the dry cycle, fishes tend to be confined in reduced habitat. The aim of this study was to evaluate the seasonal variation in trophic niche width and overlap of four species—Mylossoma duriventre, Prochilodus nigricans, Cichla.pleiozona and Serrasalmus rhombeus—in an Amazonian floodplain lake. Stable isotope analyses were used to estimate trophic niche width and overlap during the flood and dry seasons. We hypothesised broader niche width for all species during the flood cycle and a higher degree of overlap between the two piscivorous fishes during the dry cycle. Isotopic niche width was 72% broader for P. nigricans, 61% for S. rhombeus and 54% for C. pleiozona during the dry cycle, which did not support our hypothesis. Core niche width overlaps were not observed between piscivorous species in either flood or dry cycle. The results indicate that seasonal variation in isotopic niche width is specific to feeding habit. Understanding how fish trophic ecology responds to changes in the hydrological regime during the seasons is crucial for sustainable fishery management in a region where many people rely heavily on fish for nutritional and economic purposes.  相似文献